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KMID : 1123920020160010141
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Korean Journal of Oriental Physiology and Pathology
2002 Volume.16 No. 1 p.141 ~ p.145
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Effects of Gamibojungikki-tang on Total Protein Synthesis of Cultured Spinal Sensory Neurons Damaged by GLUCOSE OXIDASE
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Lee Chang-Ho
Kwon Kang-Beom
Jang Seung-Ho
Song Yung-Sun
Ryu Do-Gon
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Abstract
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In order to clarify the neuroprotective effect of Gamibojungikki-tang (GBJIKT) water extract on cultured mouse spinal sensory neuron damaged by glucose Oxidase (GO), MTT [3-(4,5-dimethylthiazole-2-yl) -2,5-diphenyltetrazolium bromide] assay and SRB (Sulforhodamine B) assay were carried out after the cultured mouse spinal sensory neuron were preincubated with various concentrations of GBJIKT water extract for 3 hours prior to exposure of GO. Cell viability of cultured mouse spinal sensory neurons exposed to various concentrations of GO for 8 hours was decreased in a dose-dependent manner. MTT50 values were 45 mU/ml GO. Cultured mouse spinal sensory neurons in the medium containing various concentration of GO for 8 hours showed decreasing of total protein synthesis. GO was toxic on cultured spinal sensory neurons. Pretreatment at GBJIKT water extract for 3 hours following GO prevented the GO-induced neurotoxicity such as decreasing of total protein synthesis. These results suggest that GO shows toxic effect on cultured spinal sensory neurons and GBJIKT water extract is highly effective in proecting the neurotoxicity induced by GO.
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KEYWORD
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Gamibojungikki-tang(加味補中益氣湯), Mouse Spinal Sensory Neuron, Glucose Oxidase, Neurotoxicity
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